Result Of Negative Staining. The acidic stain with its negatively charged chromogen will not penetrate the cells because of the negative charge on the surface of bacteria. Various factors influence the results of Gram staining.
Therefore the unstained cells are easily discernible against the colored background. Various factors influence the results of Gram staining. The negative stain uses the dye nigrosin which is an acidic dye.
The advantages of negative staining are.
A negative stain is a rapid method to observe structural features of microbes - bacteria viruses and fungi. Neural Surface Antigens 2015. Some biological elements are very small and as a result sectioning reveals aspects of its content but not about its global surface structure. Principle of Negative Staining.