Result In Increased Effective Concentration Of Substrate. The enzyme E and substrate S combine with each. Increase the concentration of the substrate and there will be increasing substrate molecules as compared to enzyme in the mixture.
The inflection point at which the increase in response with increasing ligand concentration begins to slow is the EC 50 which can be mathematically determined by derivation of the best-fit line. At higher substrate concentration the rate of reaction increase. An enzyme is supposed to speed up the reaction but our observations shows that the concentration of the substrate also had an effect on how fast the reaction could occur.
Hence if the substrate concentration is high enough the enzyme will reach the same Vmax as without the inhibitor.
This means that increasing the concentration of substrate will decrease the chance of inhibitor binding to the enzyme. When there was zero concentrate of hydrogen peroxide meaning the solvent was only water there was no reaction with the catalase. At a temperature below the optimum the molecules have less kinetic energy so the rate of collisions between enzyme and substrate molecules is low therefore less enzyme-substrate complexes are formed. Substrate Concentration Increasing substrate concentration increases the frequency with which the enzyme and substrate collide.