Group B Strep Isolated In Broth Only. Six months after insertion eight women had acquired vaginal strains and six werenolonger vaginal carriers. From a total of 580 pregnant women duplicate vaginal-rectal swabs were collected at 35 to 37 weeks of gestation and cultured for group B streptococci using either LIM broth a selective broth containing antibiotics or GBS broth for enrichment.
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Group B streptococcal bacteria can cause a wide range of illnesses in susceptible people including newborns the elderly and those with pre-existing medical conditions such as diabetes or cancer. Although Trans- Vag medium is often available without sheep blood direct comparison of medium with and without sheep blood has shown higher yield when blood is added. Vaginal swabs endocervical or introital swabs or both grew streptococci from 16 womenand23 were carrying streptococci at one or more sites sampled.
Of 124 clinical isolates 122 984 were correctly identified in less than 24 h from the selective broth medium whereas standard cultures from blood agar plates.
Because group B haemolytic streptococci are now thought important in neonatal infection the authors evaluated the broth of VINCENT et al. Agalactiae also known as group B streptococcus or GBS. A300 to detect weakly or non-hemolytic strains of GBS. It is the organism associated with strep throa.
